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Suez Canal University Medical Journal SCU-MJ


The Official Journal of Faculty of Medicine, Suez Canal University

Founded in 1998

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Current Issue Article, Volume 20, Issue 1, March 2017

Effect of Different Nanothymoquinone Concentrations on the Chemically-Induced Epithelial Dysplasia in the Hamster Buccal Pouch

Randa H. El-Sherbiny*, Magda M. Hassan, Ahmed M. Korraah

Abstract

Background: Thymoquinone (TQ), an active component of Nigella sativa, has been shown to reduce tumor growth in oral cancer models. However, toxicity at high doses with poor water solubility limits its usage as a therapeutic agent. Nanoformulations were introduced to over-come these problems. Aim: The present study was designed to evaluate the effect of different nanothymoquinone (NTQ) concentrations on the chemically-induced epithelial dysplasia of the hamster buccal pouch. Materials and Methods: NTQ was prepared to improve the effectiveness of TQ. Transmission electron microscopy confirmed the particle diameter between 6 to 15 nm. Clinical observations, histopathological alterations and complete blood count were used to in-vestigate the effect of TQ and NTQ on severe oral epithelial dysplasia induced on hamsters’ buccal pouches by 7,12-dimethylbenz[a]anthracene (DMBA). Animals received DMBA, three times weekly for six weeks then were treated with intraperitoneal injection of TQ (0.1 mg/kg) or different concentrations of NTQ (0.1, 0.05, 0.025 and 0.01 mg/kg) three times per week for another 6 weeks. Results: TQ and higher concentrations of NTQ had higher anti-tumor activity towards the severe epithelial dysplasia. The best result was with NTQ 0.1mg/kg concentration. Conclusion: NTQ is a promising candidate in cancer chemoprevention and chemotherapy as well as striated muscle regeneration. Keywords: oral cancer, DMBA, thymoquinone nanoparticles