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Suez Canal University Medical Journal SCU-MJ

The Official Journal of Faculty of Medicine, Suez Canal University

Founded in 1998

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Current Issue Article, Volume 20, Issue 2, October 2017

Evaluation of Antimicrobial Effect of Propolis, Miswak, Green Tea Compared to Sodium Hypochlorite and Chlorhexidine as Root Canal Irrigants

Abeer M. Darag, Dalia M. Fayyad, Abd El Azeem M. El Gammal, Ashraf A. Soliman*


Background: To assess the antibacterial effect of ethanolic extract of propolis, mi-swak and green tea compared to sodium hypochlorite and chlorhexidine as root canal irrigants against E.faecalis biofilm using laboratory culturing and scanning electron microscope (SEM). Materials and Methods: fifty, human, single canalled teeth were used. The crown of each tooth was sectioned. After root canal preparation and sterili-zation, the roots were infected with E. faecalis. Roots inoculated with E.faecalis were incubated at 37Co for 7 days. Then root canals were instrumented using Revo S NiTi files and different irrigants: Sodium hypochlorite 3% (NaOCl), 2% chlorhexidine (CHX), 20% ethanolic extract of Egyptian propolis, 20% ethanolic extract of Miswak and 20% ethanolic extract of Green tea. Root canals were sampled before (S1) and immediate-ly after the chemo- mechanical preparation (S2). The data obtained collected, tabu-lated, and statistically analyzed. Results: NaOCl group (2.56±2.74) showed the lowest mean Log10 of bacterial counts, it was non-statistically significant different from CHX group (3.83±2.37), Propolis group (4.62±1.88) and Miswak group (4.93±2.01). NaOCl group showed statistically significantly lower mean Log10 of bacterial counts than Green tea group (5.82±0.16) and Saline group (6.39±0.30). Conclusion: Propolis, Salva-dora Perisca and green tea offer a promising natural antimicrobial alternative and may serve as a new endodontic irrigants. Keywords: E. faecalis, herbal irrigants, root canal disinfectants