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Suez Canal University Medical Journal SCU-MJ


The Official Journal of Faculty of Medicine, Suez Canal University

Founded in 1998

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Current Issue Article, Volume 20, Issue 1, March 2017

Treatment of Oral Squamous Cell Carcinoma using Thymoquinone Loaded on Gold Nanoparticles

Merhan N. El-Mansy*, Magda M. Hassan, Kholoud M. Abou El-Nour, Wafaa H. El-Hosary

Abstract

Background: Oral cancer is one of the most prevalent cancers. Thymoquinone has a potential therapeutic activity against cancer cells. Aim: to compare the effect of thymoquinone and thy-moquinone loaded on gold nanoparticles (GNPs-TQ) as systemic therapeutic agents through intra-peritoneal injection for 3&6 weeks after painting of with DMBA 3 times/week for 14w. Material and Methods: This study was carried out on ninety male Syrian golden hamsters (n=90), its age ranged from 6 to 7 weeks, weighting 90-110 gm, purchased from Theodore Blhars Research Institute, Cairo, Egypt. Characterization of GNPs was achieved through using Ultraviolet-Visible spectroscope and transmission electron microscope (TEM). Tissue samples were examined through routine H&E stain, and nuclear factor kappa B cells (NF-κB) immuno-reactivity. Results: Formation of spherical and well dispersed gold nanoparticles (GNPs) (27nm). Histopathological evaluation showed retardation of carcinogenesis in hamster buccal pouch and regeneration of striated muscle layers when treated with thymoquinone loaded on gold nanoparticles (GNPs-TQ). These observations were in line with decrease nuclear factor-kappa B expression. Conclusions: GNPs-TQ is a promising chemo-therapeutic agent in regres-sion of carcinogenesis, and improving the general animal health. Key words: Thymoquinone, Gold nano-particles, HBP/DMBA, and NF-κB